Static magnetic field models consistent with nearly isotropic plasma pressure

Using the empirical magnetospheric magnetic field models of Tsyganenko and Usmanov (TU), we have determined the self-consistent plasma pressure gradients and anisotropies along the midnight meridian in the near-Earth magnetosphere. By “inverting” the magnetic field, we determine what distributions of an anisotropic plasma, confined within the specified magnetic field configuration, are consistent with the magnetohydrostatic equilibrium condition, J × B = ∇ · P. The TU model, parameterized for different levels of geomagnetic activity by the Kp index, provided the magnetic field values from which J × B was numerically evaluated. A best fit solution was found that minimized the average difference between J × B and ∇ · P along an entire flux tube. Unlike previous semi-empirical models, the TU models contain magnetic stresses that can be balanced by a nearly isotropic plasma pressure with a reasonable radial gradient at the equator

Anomalous aspects of magnetosheath flow and of the shape and oscillations of the magnetopause during an interval of strongly northward interplanetary magnetic field

On 15 Feb. 1978, the orientation of the interplanetary magnetic field (IMF) remained steadily northward for more than 12 hours. The ISEE 1 and 2 spacecraft were located near apogee on the dawn side flank of the magnetotail. IMP 8 was almost symmetrically located in the magnetosheath on the dusk flank and IMP 7 was upstream in the solar wind. Using plasma and magnetic field data, we show the following: (1) the magnetosheath flow speed on the flanks of the magnetotail steadily exceeded the solar wind speed by 20 percent; (2) surface waves with approximately a 5-min period and very non-sinusoidal waveform were persistently present on the dawn magnetopause and waves of similar period were present in the dusk magnetosheath; and (3) the magnetotail ceased to flare at an antisunward distance of 15 R(sub E). We propose that the acceleration of the magnetosheath flow is achieved by magnetic tension in the draped field configuration for northward IMF and that the reduction of tail flaring is consistent with a decreased amount of open magnetic flux and a larger standoff distance of the subsolar magnetopause. Results of a three-dimensional magnetohydrodynamic simulation support this phenomenological model

End of One Way

Describes the role of three South Minneapolis community-based organizations. Demonstrates how the organizations form partnerships and share leadership with their communities. Explores a set of themes derived from each example of community engagement

Utilizing the polar cap index to explore strong driving of polar cap dynamics

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/95343/1/jgra21643.pd

Identification of calcium-dependent phospholipid-binding proteins in higher plant cells

AbstractCalcium-dependent phospholipid-binding proteins of apparent Mr 33 000 and 35 000 were isolated from suspension cultures of tomato cells. Two-dimensional gel electrophoresis showed the proteins to have isoelectric points of approx. 5.7 and 5.6, respectively. In the presence of calcium, both proteins bound to liposomes formed from a mixture of phosphatidylserine and phosphatidylcholine, but not to liposomes of phosphatidylcholine alone. Both proteins showed immunological similarities to previously characterized calcium-dependent phospholipid-binding proteins (annexins) from Torpedo marmorata and mammalian species. The protein of Mr 33 000 cross-reacted with three separate antisera raised to the annexin Torpedo calelectrin, whereas that of Mr 35 000 cross-reacted with antisera to the bovine annexins p68 and p32/34. We suggest that the two proteins may represent the first identification in higher plants of the annexin family of calcium-dependent phospholipid-binding proteins

Characteristics of ion flow in the quiet inner plasma sheet

Abstract We use AMPTE/IRM and ISEE 2 data to study the properties of the high beta (βi \u3e 0.5) plasma sheet, the inner plasma sheet (IPS). Bursty bulk flows (BBFs) are excised from the two databases, and the average flow pattern in the non-BBF (quiet) IPS is constructed. At local midnight this ensemble-average flow is predominantly duskward; closer to the flanks it is mostly earthward. The flow pattern agrees qualitatively with calculations based on the Tsyganenko [1987] model (T87), where the earthward flow is due to the ensemble-average cross tail electric field and the duskward flow is the diamagnetic drift due to an inward pressure gradient. The IPS is on the average in pressure equilibrium with the lobes. Because of its large variance the average flow does not represent the instantaneous flow field. Case studies also show that the non-BBF flow is highly irregular and inherently unsteady, a reason why earthward convection can avoid a pressure balance inconsistency with the lobes. The ensemble distribution of velocities is a fundamental observable of the quiet plasma sheet flow field

Ionospheric flow shear associated with the preexisting auroral arc: A statistical study from the FAST spacecraft data

An auroral substorm is a disturbance in the magnetosphere that releases energy stored in the magnetotail into the high‐latitude ionosphere. By definition, an auroral substorm commences when a discrete auroral arc brightens and subsequently expands poleward and azimuthally. The arc that brightens is usually the most equatorward of several auroral arcs that remain quiescent for ~5 to ~60 min before the breakup commences. This arc is often referred to as the “preexisting auroral arc (PAA)” or the “growth‐phase arc.” In this study, we use FAST measurements to establish the statistics of flow patterns near PAAs in the ionosphere. We find that flow shear is present in the vicinity of a preexisting arc. When a PAA appears in the evening sector, enhanced westward flow develops equatorward of the arc, whereas when a PAA appears in the morning sector, enhanced eastward flow develops poleward of the arc. We benchmark locations of the PAAs relative to large‐scale field‐aligned currents (FACs) and convective flows in the ionosphere, finding that the arc forms in the upward current region within ~1° of the Region 1/Region 2 boundary in all local time sectors from 20 MLT to 03 MLT. We also find that near midnight in the Harang region, most of the PAAs lie within 0.5° poleward of the low‐latitude Region 1/Region 2 currents boundary and sit between the westward and eastward flow peak but equatorward of the flow reversal point. Finally, we examine arc‐associated electrodynamics and find that the FAC of the PAA is mainly closed by the north‐south Pedersen current in the ionosphere.Key PointsAn ionospheric flow shear is associated with the preexisting auroral arcThe FAC of the PAA is primarily closed by N‐S Pedersen current in the ionosphereThe PAA is located very close to the R1/R2 boundaryPeer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/112278/1/jgra51768.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/112278/2/jgra51768-sup-0001-supinfo.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/112278/3/jgra51768-sup-0002-supinfo.pd

Protease-activated receptor 4 variant p.Tyr157Cys reduces platelet functional responses and alters receptor trafficking

OBJECTIVE—: Protease-activated receptor 4 (PAR4) is a key regulator of platelet reactivity and is encoded by F2RL3, which has abundant rare missense variants. We aimed to provide proof of principle that rare F2LR3 variants potentially affect on platelet reactivity and responsiveness to PAR1 antagonist drugs and to explore underlying molecular mechanisms. APPROACH AND RESULTS—: We identified 6 rare F2RL3 missense variants in 236 cardiac patients, of which the variant causing a tyrosine 157 to cysteine substitution (Y157C) was predicted computationally to affect most on PAR4 structure. Y157C platelets from 3 cases showed reduced responses to PAR4-activating peptide and to α-thrombin compared with controls, but no reduction in responses to PAR1-activating peptide. Pretreatment with the PAR1 antagonist vorapaxar caused lower residual α-thrombin responses in Y157C platelets than in controls, indicating greater platelet inhibition. HEK293 cells transfected with a PAR4 Y157C expression construct had reduced PAR4 functional responses, unchanged total PAR4 expression but reduced surface expression. PAR4 Y157C was partially retained in the endoplasmic reticulum and displayed an expression pattern consistent with defective N-glycosylation. Mutagenesis of Y322, which is the putative hydrogen bond partner of Y157, also reduced PAR4 surface expression in HEK293 cells. CONCLUSIONS—: Reduced PAR4 responses associated with Y157C result from aberrant anterograde surface receptor trafficking, in part, because of disrupted intramolecular hydrogen bonding. Characterization of PAR4 Y157C establishes that rare F2RL3 variants have the potential to markedly alter platelet PAR4 reactivity particularly after exposure to therapeutic PAR1 antagonists

Notch ligation by Delta1 inhibits peripheral immune responses to transplantation antigens by a CD8⁺ cell–dependent mechanism

Notch signaling plays a fundamental role in determining the outcome of differentiation processes in many tissues. Notch signaling has been implicated in T versus B cell lineage commitment, thymic differentiation, and bone marrow hematopoietic precursor renewal and differentiation. Notch receptors and their ligands are also expressed on the surface of mature lymphocytes and APCs, but the effects of Notch signaling in the peripheral immune system remain poorly defined. The aim of the studies reported here was to investigate the effects of signaling through the Notch receptor using a ligand of the Delta-like family. We show that Notch ligation in the mature immune system markedly decreases responses to transplantation antigens. Constitutive expression of Delta-like 1 on alloantigen-bearing cells renders them nonimmunogenic and able to induce specific unresponsiveness to a challenge with the same alloantigen, even in the form of a cardiac allograft. These effects could be reversed by depletion of CD8⁺ cells at the time of transplantation. Ligation of Notch on splenic CD8⁺ cells results in a dramatic decrease in IFN-γ with a concomitant enhancement of IL-10 production, suggesting that Notch signaling can alter the differentiation potential of CD8⁺ cells. These data implicate Notch signaling in regulation of peripheral immunity and suggest a novel approach for manipulating deleterious immune responses